质粒名称：NL4-3 mCherry Luciferase
质粒编号：44965
质粒备注：

HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

载体名称：pNLENG1
完整质粒大小：12424
载体类型：慢病毒
筛选标记：mCherry, Luciferase

原核抗性：氨苄青霉素，100μg/mL
培养温度：30℃
克隆菌株：Stbl3
高拷贝/低拷贝：未知

插入基因：mCherry, Luciferase, IRES
基因别名：HIV 1 reporter virus
物种来源：Lentivirus, Human specific
基因突变：S34C mutation in Nef
启动子：EF-1 alpha– driven mCherry:Puromycin
标签/融合蛋白：· mCherry with D212G (C terminal on insert) · Luciferase, IRES (C terminal on insert)

克隆方法：限制性内切酶
5'克隆位点：BamHI (not destroyed)
3'克隆位点：XhoI (destroyed during cloning)
5'测序引物：from pNLENG1 5'
3'测序引物：from pNLENG1 3'

质粒图谱：