质粒名称：VVT1
质粒编号：65779
质粒备注：

The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

载体名称：MLM3636
载体类型：哺乳动物表达，CRISPR

原核抗性：氨苄青霉素，100μg/mL
培养温度：37℃
克隆菌株：XL1 Blue
高拷贝/低拷贝：高拷贝

插入基因：SaCas9 gRNA backbone, without spacer sequence
物种来源：Synthetic
启动子：U6

克隆方法：Gibson Cloning
5'测序引物：OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')

质粒图谱：