质粒名称：pYLCRISPR/Cas9Pubi-H
质粒编号：66187
质粒备注：

expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

载体名称：pCAMBIA1300
载体类型：Plant Expression
筛选标记：潮霉素

原核抗性：卡那霉素，50μg/mL
培养温度：37℃
克隆菌株：Top10F'
高拷贝/低拷贝：未知

插入基因：Cas9
基因突变：plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content
启动子：maize ubiquitin promoter (Pubi)

克隆方法：未知
5'测序引物：M13pUC-Rev
3'测序引物：NOSterm-R attgccaaatgtttgaacga

质粒图谱：