Smilagenin

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Smilagenin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

126-18-1

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
50mg	电议
100mg	电议
200mg	电议
500mg	电议

产品介绍

Smilagenin (SMI) 是一种小分子甾体皂苷元，来自知母Anemarrhena asphodeloides和天竺葵Pelargonium hortorum，广泛用于慢性神经退行性疾病的研究。Smilagenin (SMI) 通过增加毒蕈碱受体亚型 1 (M1)-受体的的密度来改善老年大鼠的记忆。 Smilagenin (SMI) 通过刺激脑源性神经营养因子的基因表达减弱 Aβ (25-35) 诱导的神经变性，可用于 AD 的研究。

生物活性

Smilagenin (SMI) is a small-molecule steroidal sapogenin fromAnemarrhena asphodeloidesandPelargonium hortorumwidely used in traditional Chinese medicine for treating chronic neurodegeneration diseases^[1]. Smilagenin (SMI) improves memory of aged rats by increasing the muscarinic receptor subtype 1 (M1)-receptor density^[2]. Smilagenin (SMI) attenuates Aβ(25-35)-induced neurodegenerationvia stimulating the gene expression of brain-derived neurotrophic factor, may represents a novel therapeutic strategy for AD^[3].

IC₅₀& Target

mAChR1

Human Endogenous Metabolite

体外研究
(In Vitro)

Smilagenin (10 μM; 24 hours) increases SH-SY5Y cell survival compared with Aβ(25-35) intoxicated cells^[3].
Smilagenin (10 μM; 24 hours) increases neurotrophic factor (GDNF) and neurotrophic factor (BDNF) mRNA level by promoting CREB phosphorylation in 1-methyl-4-phenylpyridimium (MPP⁺) treated SH-SY5Y cells^[2].

Cell Viability Assay^[3]

Cell Line:	SH-SY5Y cells
Concentration:	10 μM
Incubation Time:	24 hours
Result:	Elevated the SH-SY5Y cell viability.

RT-PCR^[2]

Cell Line:	SH-SY5Y cells
Concentration:	10 μM
Incubation Time:	24 hours
Result:	Increased GDNF and BDNF transcription.

体内研究
(In Vivo)

Smilagenin (intragastric administration; 10 or 26 mg/kg, once daily; 60 days) prevents the impairment of dopaminergic neurons in chronic MPTP/probenecid-induced mouse model^[2].

Animal Model:	MPTP/probenecid-induced mouse model^[2]
Dosage:	10 or 26 mg/kg
Administration:	Intragastric administration; 10 or 26 mg/kg; once daily; 60 days
Result:	Ameliorated locomotor ability of MPTP/probenecid-lesioned mice.

分子量

416.64

性状

Solid

Formula

C₂₇H₄₄O₃

CAS 号

126-18-1

结构分类

Steroids

来源

Plants
Geraniaceae
Pelargonium hortorumBailey

Plants
Liliaceae
Anemarrhena asphodeloidesBunge

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

Ethanol : ≥ 10 mg/mL(24.00 mM)

DMSO : 5 mg/mL(12.00 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.4002 mL	12.0008 mL	24.0015 mL
5 mM	0.4800 mL	2.4002 mL	4.8003 mL
10 mM	0.2400 mL	1.2001 mL	2.4002 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。