| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 100mg | 电议 |
| 1g | 电议 |
| 5g | 电议 |
| 50g | 电议 |
| 100g | 电议 |
Cell lines | Glioma Cells：LNT-229, G55, and U343 glioma cells |
Preparation Method | Doxycycline hyclate was dissolved in water (stock 1 mg/mL). The following concentrations were analyzed: 0.0, 0.1, 1, 10 g/mL. Cell growth using crystal violet staining (CV) was measured after incubation under standard conditions. |
Reaction Conditions | Doxycycline hyclate 0.01, 0.1, 1 or 10 μg/mL for 4 days |
Applications | Doxycycline hyclate affected growth of glioma cells only under high concentrations (10 μg/mL). |
Animal models | Mouse model of P. berghei malaria (Female Swiss Webster mice average of 20 g body weight) |
Preparation Method | Doxycycline hyclate 10 mg/kg and 50 mg/kg once daily; dissolved in PBS in a total volume of 100 μl; intraperitoneally |
Dosage form | 10 mg/kg and 50 mg/kg, 4days |
Applications | In mouse model of P. berghei malaria, Doxycycline hyclate exhibited potent antimalarial activity at 50 mg/kg and activity was suboptimal at 10 mg/kg. |
| 产品描述 | Doxycycline hyclate is a derivative of tetracycline and possesses the activities of anti-inflammatory and antimicrobial. Doxycycline hyclate is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[5].Doxycycline hyclate treatment reduces MMP-8 and -9 levels and inhibits expression of tissue MMP-2 and MMP-9. Moreover, treatment with doxycycline hyclate significantly reduces the incidence of intracranial aneurysms. Doxycycline hyclate has also been reported to be an anti-inflammatory agent based on its inhibition of matrix metalloproteinases. In addition, doxycycline has potent antimalarial activity with IC50value of 320nM at 96h in vitro[2 3]. Doxycycline hyclate inhibits dengue virus replication in vitro with a temperature-dependent manner. The IC50value is 52.3μM at 37℃ and 26.7μM at 40℃. It inhibits the dengue virus via inhibiting the NS2B-NS3 serine protease of the virus[5] The ability of doxycycline hyclate to cause severe mitonuclear protein imbalance in glioma and astroglial cells, especially at high concentrations. Doxycycline hyclate had no effect on cell growth at concentrations regularly used to induce gene expression. Only the highest tested concentration of doxycycline hyclate (10 ug/mL) impaired cell growth[4].In human cell lines, commonly used concentrations of doxycycline hyclate change gene expression patterns and concomitantly shift metabolism towards a more glycolytic phenotype, evidenced by increased lactate secretion and reduced oxygen consumption[7].Doxycyline can inhibit the viability and proliferation of breast cancer cells and BCSCs, decrease mammosphere forming efficiency, migration and invasion, and EMT of breast cancer cells. Expression of stem cell factors were also significantly downregulated after doxycycline hyclate treatment[8] In mouse model of P. berghei malaria, Doxycycline hyclate exhibited potent antimalarial activity at 50 mg/kg and activity was suboptimal at 10 mg/kg[1].Doxycycline hyclate treatment inhibits the activity of tissue MMP and attenuates the decrease in the collagen content in aortas of mice haploinsufficient for collagen III, as well as prevents the development of stress-induced vessel pathology. The results suggest that doxycycline hyclate merits clinical testing as a treatment for vEDS(The vascular form of Ehlers-Danlos syndrome)[6] References: |
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