Estradiol 是一种类固醇性激素，对于女性生育能力和第二性征的维持至关重要。它通过雌激素受体 β 途径上调 IL-6 表达。

Estradiol is an Estrogen. The mechanism of action of estradiol is as an Estrogen Receptor Agonist.

Estradiol可以逆转卵巢切除引发的脊椎密度下降.Estradiol调节成年大鼠发情周期内海马突触密度的波动.

Estradiol诱导海马CA1锥体细胞产生新的树突棘和突触。Estradiol处理导致CA1区锥体细胞对NMDA受体介导的突触输入的敏感性增加,而这种增加与这些细胞的顶树突树中Estradiol诱导树突棘密度的增加有很好的相关性。在急性分离和培养的纹状体神经元中,17 beta-estradiol可逆地通过钡离子通道抑制钙离子进入。17 alpha-estradiol也减少了钡离子电流,但是明显比17 beta-estradiol对大鼠纹状体神经元的效果较差。17 beta-estradiol剂量依赖性抑制IL-1,TNF-α和IL-1和TNF-α诱导的IL-6的产生。Estradiol抑制新生小鼠颅盖骨来源的原骨细胞培养物中TNF-α诱导的IL-6的产生和破骨细胞的生成。

Estradiol is dissolved in 0.01% ethanol. Briefly, B9 cells (5×103/well of a 96-well plate) are cultured with a series of dilutions of the supernatants in a final volume of 100 mL of RPMI 1640, supplemented with 5×10-5mol/Lof 2-mercaptoethanol, 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin in flat-bottom microtiter plates. After 42 h, 0.5 μCi of [3H]thymidine is added. 6 h later, the cells are harvested and the radioactivity incorporated is determined. IL-6 is quantitated from a standard curve set up with known amounts of recombinant human or mouse IL-6. The anti-IL-6 monoclonal antibody completely inhibited the ability of the B9 cells to proliferate in response to recombinant mouse IL-6. In addition, we determined that B9 cells do not proliferate in response to IL-I or TNF, and that antibodies to these two cytokines did not affect the cell proliferation in response to IL-6. Further, it is established that neither Estradiol (17β-estradiol), nor the 0.01% ethanol used as vehicle in these experiments, has any effect on the bioassay.

50-28-2

C18H24O2

272.388

雌二醇;β-Estradiol;E2;17β-Estradiol;17β-Oestradiol

DMSO:27.2 mg/mL (100 mM)
Ethanol:20.4 mg/mL (75 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years