FGFR4-IN-1

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FGFR4-IN-1

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	1708971-72-5
包装:	5mg, 10mg

包装与价格：

产品介绍

FGFR4-IN-1是一种有效的选择性FGFR4抑制剂，IC50 值为 0.7 nM。FGFR4-IN-1显著抑制HuH-7肝细胞癌细胞的增殖，IC 50为7.8 nM。FGFR4可能会成为治疗胃癌的新型靶标。

细胞实验
细胞系	HuH-7 hepatocellular carcinoma cells
方法	FGFR4-IN-1 is dissolved in DMSO. Methylene blue staining proliferation assay (MBS): The effect of compounds on cell proliferation is assessed using HuH-7 hepatocellular carcinoma cells are cultured in the vendor-recommended medium, 10% foetal calf serum, 1 mM sodium pyruvate, 1x Penicillin/Streptomycin at 37°C in a humidified 5% CO2 incubator. Specifically, 5000 cells/well are seeded in 96-well tissue culture plates in a total media volume of 100 μL/well and increasing compound dilutions or DMSO are added 24 hours thereafter in triplicates. 72 hours after compound addition, cells are fixed by adding 25 and incubated for 10 minutes at room temperature. Cells are washed three times with H2O. Cells are washed 3 times with H2O, 200 mL/well and then lysed by adding 200 mL/well of 3% HCl for 30 minutes at room temperature with shaking. Optical density is measured at A650 nm. The concentration of compound providing 50% of proliferation inhibition with respect to DMSO-treated cells is determined (IC50) using XLFit software.
浓度
处理时间	72 hours

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.0263 mL	10.1313 mL	20.2626 mL
5 mM	0.4053 mL	2.0263 mL	4.0525 mL
10 mM	0.2026 mL	1.0131 mL	2.0263 mL