| 包装 | 价格(元) |
| 5g | 电议 |
| 25g | 电议 |
Animal experiment: | Mice: Male Sprague Dawley rats (150-200g) are used in the study. LDABA is dissolved in 09.% saline and diluted in appropriate medium. L-DABA is administered intraperitoneally at a dose of 764 mg/kg in a volume of 4 mL/kg in acute studies. Chronically treated rats receives daily intraperitoneally injections (2.5mM/kg in saline) for 3 days. Mice are sacrificed and the brain regions are dissected for analysis[2]. |
| 产品描述 | L-DABA (L-2,4-Diaminobutyric acid) is a week GABA transaminase inhibitor with an IC50 of larger than 500 μM; exhibits antitumor activity in vivo and in vitro. The tumor cells are irreversibly and totally damaged by incubation with 10 mM L-2,4-Diaminobutyric acid for 24 h at 37℃. The cell-destructive effect by L-DABA is probably due to an osmotic lysis induced by the non-saturated intracellular accumulation of L-DABA. The harmful effect of L-DABA could be abolished by concomitant incubation with L-alanine and L-methionine[1]. Kinetic studies indicates that L-DABA is a non-linear, non-competitive inhibitor of GABA transaminase activity. The L-DABA-induced elevation of GABA levels parallels the inhibition of GABA transaminase activity[2]. L-2,4-Diaminobutyric acid, an amino acid analogue, produceS a cytolytic effect with a human glioma cell line, SKMG-1, and normal human fibroblasts. The concentrations of L-DABA necessary to reduce the cell count to 50% of control following a 24-h incubation at 37℃ are 12.5 mM for the human fibroblasts and 20 mM for the glioma cell line[3]. Treatment with L-DABA results in 43.4% reduction of tumor growth[1]. L-DABA is a more effective inhibitor of GABA transaminase in vivo than in vitro[2]. References: |
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