Cy3 Bis NHS ester

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Cy3 Bis NHS ester

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
5mg	电议
25mg	电议

产品介绍

化学性质

Storage	Store at -20°C
M.Wt	932.99
Formula	C₄₃H₄₉N₄NaO₁₄S₂
Solubility	insoluble in EtOH; ≥41 mg/mL in DMSO; ≥54.6 mg/mL in H2O
Chemical Name	sodium 1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-((1E,3E)-3-(1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
Canonical SMILES	CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CCCCCC(ON3C(CCC3=O)=O)=O)(C)C2=C/C=C/C4=[N+](CCCCCC(ON5C(CCC5=O)=O)=O)C(C=CC(S(=O)([O-])=[O-])=C6)=C6C4(C)C.[Na+]
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Cy3 Bis NHS ester is a reactive fluorescent dye for general labeling of protein amine groups. Cy3 Bis NHS ester includes two reactive groups on one dye molecule. The N-hydroxysuccinimde (NHS) ester is the most popular amine reactive group, used for capturing the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Appling NHS chemistry allows one to conjugate a variety of fluorescent probes (e.g. Cy3, a traditional orange-fluorescent label which is highly water soluble) to primary amines. Thus, target biological compounds can be visualized by Cy3 fluorescence (~550 nm excitation, ~570 nm emission) monitored by various fluorometers, imagers, and microscopes.

References:

1. Nanda JS, Lorsch JR. Labeling a protein with fluorophores using NHS ester derivitization. Methods in Enzymology, 2014, 536: 87-94.

2. Dyla M, Andersen JL, Kjaergaard M, et al. Engineering a Prototypic P-type ATPaseListeria monocytogenesCa²⁺-ATPase 1 for Single-Molecule FRET Studies. Bioconjugate Chemistry, 2016, 27(9): 2176-2187.