Norartocarpetin

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Norartocarpetin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

520-30-9

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议
25mg	电议

产品介绍

Norartocarpetin 是一种酪氨酸酶抑制剂。Norartocarpetin 具有较强的酪氨酸酶抑制活性，其抑制活性IC₅₀值为 0.47 μM。Norartocarpetin 作为一种抗褐变剂可用于食品体系的研究。Norartocarpetin 对肺癌细胞 (NCI-H460) 也具有显著的抗癌活性，其IC₅₀值为 22 μM。Norartocarpetin 的抗增殖作用是通过靶向 Ras/Raf/MAPK 信号通路、线粒体介导的细胞凋亡、S 期细胞周期阻滞和抑制细胞迁移和侵袭实现的。

生物活性

Norartocarpetin is atyrosinaseinhibitor. Norartocarpetin has strongtyrosinaseinhibitory activity with anIC₅₀value of 0.47 μM. Norartocarpetin as an antibrowning agent can be used for the research of food systems. Norartocarpetin also has a significant anticancer activity in lung carcinoma cells (NCI-H460) with anIC₅₀value of 22 μM. Norartocarpetin has antiproliferative effects are mediated via targeting Ras/Raf/MAPK signalling pathway, mitochondrial mediatedapoptosis, S-phase cell cycle arrest and suppression of cell migration and invasion in human lung carcinoma cells^[1]^[2].

IC₅₀& Target

IC50: 0.47 μM (tyrosinase);^[1]IC50: 22 μM (NCI-H460 cells); 85 μM (MRC-9 cells)^[2]

体外研究
(In Vitro)

Norartocarpetin has strong mushroom tyrosinase inhibitory activity with anIC₅₀value of 0.47 μM^[1].
Norartocarpetin (0-100 μM; 48 h) leds to dose-dependent cytotoxic effects in NCI-H460 cells and MRC-9 cells with IC₅₀values of 22 μM and 85 μM, respectively^[2].
Norartocarpetin (0, 11, 22, and 44 μM; 24 h) led to blockade of Ras/Raf/MAPK signalling pathway^[2].
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) induced the apoptosis of the human lung carcinoma cells (NCI-H460)^[2].
Norartocarpetin (0, 11, 22, and 44 μM) leds to S-phase cell cycle arrest^[2].
Norartocarpetin (22 μM; 24 h) leds to dose-dependent suppression of cell invasion^[2].
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) leds to significant inhibition of cell migration^[2].

Cell Viability Assay^[2]

Cell Line:	MRC-9 (normal fibroblast lung cells) and NCI-H460 (human lung cancer cells)
Concentration:	0, 3.12, 6.25, 12.5, 25, 50, and 100 μM (first dissolved in DMSO at 1.0 mg/mL and then diluted to different concentrations using DMSO, 30 μL, 20min)
Incubation Time:	48 h
Result:	Repressed the viability of NCI-H460 cells in a dose-dependent manner.

Western Blot Analysis^[2]

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0, 11, 22, and 44 μM
Incubation Time:	24 h
Result:	Significantly reduced the expression of p-RAS, p-RAF and p-P38 and unchanged the expressions of RAS, RAF and P38.

Apoptosis Analysis^[2]

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0. 11, 22 and 44 μM
Incubation Time:	24 h
Result:	Led to dose-dependent apoptosis and induced DNA damage.

Cell Cycle Analysis^[2]

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0, 11, 22, and 44 μM
Incubation Time:
Result:	Showed increase of S-phase cells and the percentage of S-phase NCI-H460 LC cells was 30%, 40%, 55% and 70% at 0, 11, 22 and 44 μM concentrations, respectively.

Cell Invasion AssayMRC-9 and NCI-H460 cells

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	22 μM
Incubation Time:	24 h
Result:	Significantly reducted (from 100% to about 40%) in the invasion of NCI-H460 human LC cells.

Cell Migration Assay^[2]

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0, 11, 22, and 44 μM
Incubation Time:	24 h
Result:	Decreased remarkably the cell invasion in NCI-H460 LC cells in a concentration- dependent manner.

分子量

286.24

性状

Solid

Formula

C₁₅H₁₀O₆

CAS 号

520-30-9

结构分类

Flavonoids
Flavones

来源

Plants
Moraceae

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(349.36 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.4936 mL	17.4679 mL	34.9357 mL
5 mM	0.6987 mL	3.4936 mL	6.9871 mL
10 mM	0.3494 mL	1.7468 mL	3.4936 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。