Stains-All, a cationic carbocyanine dye, is a convenient probe to study the structural features of the individual calcium-binding sites ofcalmodulin(CaM) and related calcium-binding proteins (CaBP)^[1][2].

Almost all of the proteins found in skeletal muscle extracts, including the Ca²⁺+Mg²⁺-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca²⁺-binding proteins blue, Stains-all is a more sensitive stain for these Ca²⁺-binding proteins than is Coomassie blue^[3].

559.58

Solid

C₃₀H₂₇BrN₂S₂

7423-31-6

着色剂-ALL

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

DMSO : 8.33 mg/mL(14.89 mM;ultrasonic and warming and heat to 80℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。