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LCS-1
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
LCS-1图片
CAS NO:41931-13-9
包装与价格：
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
LCS-1 是一种超氧化物歧化酶 1 (SOD1) 抑制剂。LCS-1 抑制 SOD1 活性，IC50值为 1.07 μM。LCS-1 诱导多发性骨髓瘤细胞 (MM.1S) 的早期和晚期凋亡(apoptosis)。
生物活性

LCS-1 is a superoxide dismutase 1 (SOD1) inhibitor. LCS-1 inhibits SOD1 activity with anIC50value of 1.07 μM. LCS-1 induces the early- and late-stageapoptosisof multiple myeloma (MM.1S) cells[1][2][3].

IC50& Target[2]

SOD1

1.07 μM (IC50)

体外研究
(In Vitro)

LCS-1 (1-10000 nM; 24 hours) has selective cytotoxicity towards bloom syndrome gene product (BLM) -proficient and BLM-deficient HCT116 cells[1].
LCS-1 shows growth inhibitory effect on 10/27 adenocarcinoma cell lines (median IC50=0.20 μM; such as H23, H2347, HCC827 cell lines) and normal human bronchial epithelial (NHBE) cells (IC50=2.66 μM)[2].
LCS-1 (0, 1.25, 2 μM; 4 h) in a concentration-dependent manner triggers significant inhibition of SOD1 enzymatic activity in multiple myeloma (MM) cells[3].
LCS-1 (0, 1.25, 2.5, 5 μM; 48 h) in a dose-dependent manner reduces the viability of various MM cell lines, including MM.1R (Dexamethasone-resistant), Dox40 (Doxorubicin-resistant), or LR5 (Melphalan-resistant) cell lines[3].
LCS-1 (48 h) has IC50values of 2.5 and 4.6 μM for cell viability of ANBL6-WT (Bortezomib-sensitive) and ANBL6-BR (Bortezomib- resistant) cells, respectively[3].
LCS-1 (1.25 μM; 16 h) induces a significant increase in ROS levels and O2–levels in MM.1S cells[3].
LCS-1 (1.25 μM; 16 h) shows a significant decrease in GSH/GSSG ratio in MM.1S cells[3].
LCS-1 (1.25 μM; 24h) induces the release of mitochondrial cytochrome-c into the cytosol, and enriches the proteins (HSP60/CLPP) mediating mtUPR signaling in MM.1S cells[3].
LCS-1-induced O2–(1.25 μM; 5 h) triggers a marked decrease in both RP2CP and RP1CP forms of 26S proteasomes[3].
LCS-1 (2 μM; 16 h) induces the early- and late-stage apoptosis of MM.1S cells[3].
LCS-1 (0, 0.5, 1, 1.5, 2 μM) upregulates p53/p21 signaling, as well as downregulates survival pathway proteins MCL-1, BclxL, or c-Myc in MM.1S cells[3].
LCS-1 (0, 4, 8, 16, 24 h; 2 μM) shows a rapid and robust induction of mitochondrial unfolded protein response (UPR) proteins (BIP, PERK, phosphorylated eIF2α, or a lectin protein calnexin) in MM.1S and ANBL6-BR cells[3].

Cell Viability Assay[1]

Cell Line:BLM-proficient and BLM-deficient HCT116 cells
Concentration:1-10000 nM
Incubation Time:24 hours
Result:Had IC50values of 1462 nM and 24.92 nM for the viability of BLM-proficient and BLM-deficient HCT116 cells, respectively.

Western Blot Analysis[3]

Cell Line:MM.1S and ANBL6-BR cells
Concentration:2 μM
Incubation Time:16 hours
Result:Decreased the expression of cell-cycle regulatory proteins (cyclin-B1, CDC25C, and CDC2).

Western Blot Analysis[3]

Cell Line:MM.1S cells
Concentration:0, 0.5, 1, 1.5, 2 μM
Incubation Time:
Result:Upregulated p53/p21 signaling, as well as downregulated survival pathway proteins MCL-1, BclxL, or c-Myc.

Western Blot Analysis[3]

Cell Line:MM.1S cells
Concentration:2 μM
Incubation Time:0, 4, 8, 16, 24 hours
Result:Showed a rapid and robust induction of UPR proteins (BIP, PERK, phosphorylated eIF2α, or a lectin protein calnexin).
体内研究
(In Vivo)

LCS-1 (20 mg/kg; i.p. every other day for 14 days) inhibits MM growth and prolongs host survival in MM.1S-bearing mice[3].

Animal Model:5-week-old female CB17 SCID mice (MM.1S tumors volume=100 mm3)[3]
Dosage:20 mg/kg (diluted in saline)
Administration:Intraperitoneal injections; treated on an every other day schedule for 14 days
Result:Inhibited MM growth and prolongs host survival.
分子量

255.10

性状

Solid

Formula

C11H8Cl2N2O

CAS 号

41931-13-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 50 mg/mL(196.00 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.9200 mL19.6002 mL39.2003 mL
5 mM0.7840 mL3.9200 mL7.8401 mL
10 mM0.3920 mL1.9600 mL3.9200 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (9.80 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.80 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在本网站选购。